Activated Silk™ 33B-α Bioactive Peptide

Better skin barrier.
Better biotechnology.
Better future.

INCI: Soluble Fibroin

  • Clinically Proven
  • Sustainable†
  • Upcycled
  • Biodegradable
  • Naturally Derived

†The manufacturing of Activated Silk™ biotechnology is environmentally responsible and developed with good work practices, which contributes to achieving U.N. Sustainable Development Goals. For more information, visit sdgs.un.org/goals

Activated Silk™ 33B-α Bioactive Peptide

Activated Silk™ 33B-α is a bioactive peptide solution that boosts the skin barrier. An improved barrier locks in moisture and locks out instigators that can lead to inflammation. So skin looks and feels healthier.

INCI: Soluble Fibroin

Activated Silk™ 33B-α:

  • An ingredient that firms and hydrates the skin while preventing signs of aging such as fine lines and wrinkles.
  • A non-occlusive ingredient that boosts the skin’s ability to maintain its own barrier against instigators that could lead to inflammation.
  • A water soluble peptide that works in places ceramides & petrolatum cannot.

Activated Silk™ 33B-α Bioactive Peptide

We have spent 10 years innovating Activated Silk™ biotechnology on a mission to promote the health of people and the planet.

INCI: Soluble Fibroin

Upcycled. Renewable.
Cruelty-free. Sustainable.†

  1. Mulberry plants are grown on 6 of 7 continents, supporting good soil, fixing erosion, and sequestering carbon.
  2. Mulberry farms provide decent work, economic growth and inclusive employment.
  3. Only a portion of the plant’s mulberry leaves are needed to feed silkworms.
  4. Silkworms spin cocoons of natural silk fiber and de-differentiate into pupae.
  5. Silkworm pupae become a vital food source for various animals, icnluding chicken and fish.
  6. Cocoons are upcycled for biotech development rather than discarded as industrial waste.
  7. Evolved By Nature uses renewable energy and is an equitable employment company.

†The manufacturing of Activated Silk™ biotechnology is environmentally responsible and developed with good work practices, which contributes to achieving U.N. Sustainable Development Goals. For more information, visit sdgs.un.org/goals

Better Skin Barrier.

Collagen plumps the underlying skin layers, but Claudin is the protein that literally binds the top layer of skin together. Without it, skin barrier cells become looser and drift apart, creating less tight junctions, which can lead to inflamed unhealthy skin and the visible signs of aging.

Because without tight junctions, more environmental stressors can come into the skin and more hydration can escape the skin, creating a vicious cycle of unhealthy inflamed looking skin.

INCI: Soluble Fibroin

Fig. 1 - Without Claudin-1
Fig. 2 - With Claudin-1 Enforced Tight Junctions

Better Biotechnology.

Activated Silk™ 33B-α is a bioactive peptide that is clinically proven to help the skin barrier look and feel healthier. A better barrier keeps out instigators, locks in hydration, calms the appearance of irritation and redness, limits the appearance of inflammation and promotes firmer, younger looking skin.

INCI: Soluble Fibroin

  • Activated Silk™ 33B-α
  • Claudin-1
  • Activated Silk™ 33B-α
  • Cell Body
  • DNA
Fig. 1 - Non-occlusive Activated Silk™ 33B-α Fig. 2 - Activated Silk™ 33B-α working to firm and support natural production of Claudin-1 Fig. 3 - Claudin-1 restored to healthy levels
  1. Non-occlusive Activated Silk™ 33B-α Fig.1
  2. Activated Silk™ 33B-α working to firm and support natural production of Claudin-1 Fig.2
  3. Claudin-1 restored to healthy levels Fig.3

Better Results.

These are the results from a study we performed to evaluate the skin barrier before and after the application of Activated Silk™ 33B-α. Activated Silk™ 33B-α bioactive peptides increased natural production of Claudin-1.

INCI: Soluble Fibroin

Barrier Enhancement
Ex-Vivo Human Skin Study Insulted skin 24hrs after control and test treatments

Claudin-1 Cell Nuclei

 

Fig 1. No Activated Silk™ 33B-α

Fig 2. Activated Silk™ 2% 33B-α

Better Results.

We conducted a head to head study versus a placebo control against major anti-aging indications.

  • Remarkable reduction in TEWL (Transepidermal water loss)
  • Remarkable reduction in the appearance of redness
  • Remarkable reduction in the appearance of wrinkles/fine lines

INCI: Soluble Fibroin

ACTIVATED SILK™ 33B-α vs
PLACEBO CONTROL

MODEL: Improvement of skin health

TEST METHOD: 54 subjects

MATERIALS: Serum (Placebo); Serum (2% Activated Silk™ 33B-α)

LENGTH OF THE STUDY: 28 days

INSTRUMENT(S): Corneometer, Cutometer, Tewameter

  1. TEWL
  2. Redness
  3. Wrinkles

NOTE:

Activated Silk™

  • 34 subjects (female)
  • Ages: 31–65
  • Race: 82.4% White, 8.8% Indigenous, 8.8% Asian (no Fitzpatrick breakdown)

Placebo

  • 23 subjects
  • Ages: 38–63
  • Race: 69.6% White, 21.7% Other, 8.7% Asian (no Fitzpatrick breakdown)

Better Results.

We conducted a head to head study versus a placebo control against major anti-aging indications.

  • Remarkable reduction in TEWL (Transepidermal water loss)
  • Remarkable reduction in the appearance of redness
  • Remarkable reduction in the appearance of wrinkles/fine lines

INCI: Soluble Fibroin

ACTIVATED SILK™ 33B-α vs
PLACEBO CONTROL Expert Graded

Results Significant reduction in TEWL (transepidermal water loss) by 18%

  1. TEWL
  2. Redness
  3. Wrinkles

Better Results.

We conducted a head to head study versus a placebo control against major anti-aging indications.

  • Remarkable reduction in TEWL (Transepidermal water loss)
  • Remarkable reduction in the appearance of redness
  • Remarkable reduction in the appearance of wrinkles/fine lines

INCI: Soluble Fibroin

ACTIVATED SILK™ 33B-α vs
PLACEBO CONTROL Expert Graded

Results Appearance of redness was significantly reduced in as little as 7 Days

  1. TEWL
  2. Redness
  3. Wrinkles

Better Results.

We conducted a head to head study versus a placebo control against major anti-aging indications.

  • Remarkable reduction in TEWL (Transepidermal water loss)
  • Remarkable reduction in the appearance of redness
  • Remarkable reduction in the appearance of wrinkles/fine lines

INCI: Soluble Fibroin

ACTIVATED SILK™ 33B-α vs
PLACEBO CONTROL Expert Graded

Results Reduction in appearance of fine lines & wrinkles.

  1. TEWL
  2. Redness
  3. Wrinkles

Better Formulation Flexibility.

We proudly offer two different Activated Silk™ 33B-α products optimized for your formulation needs.

INCI: Soluble Fibroin

  1. Activated Silk™ 33B-α 113 for mid viscosity formulations.
  2. Activated Silk™ 33B-α 720 for low viscosity formulations.

Better Formulation Flexibility.

INCI: Soluble Fibroin

Anchor ingredient in wide array of finished formula applications

  • Formulas that are incompatible with ceramides / petrolatums / typical barrier ingredients
  • All products can work in spray application, something ceramides can’t do

Better Shipping.

Activated Silk™ 33B-α is delivered in a lyophilized tablet form. Why? Because it makes it easier to ship, store and use. Which means it’s better for your business, budget and footprint.

INCI: Soluble Fibroin

When compared to a standard liquid, waxy or petrolatum ingredients, Activated Silk™ 33B-α:

  • Reduces the shipping burden by 90.5%*
  • Reduces plastic packaging by 56%*
  • Renewably sourced, Upcycled and biodegradable making it supportive of a circular economy
  • Is compact for easy storage Compact

*Compared to standard 55 gallon drum liquid ingredient

Better Retention.

Activated Silk™ 33B-α is more than a facial barrier boosting ingredient. We ran retention studies to prove that the bioactive peptide stays on the skin in rinse-off products, making it a revolutionary barrier ingredient for a wide array of below-the-neck formulations as well.

INCI: Soluble Fibroin

Expanding Commercial Applications

  • Body Cream
  • SPF Products
  • Body Cleansing Products
  • Body Sprays & Deodorants

Better Retention.

Skin samples were washed with body wash including Activated Silk™ 33B-α for 15 minutes and then rinsed. Skin samples were then examined for retention of the Activated Silk™ 33B-α bioactive peptide ingredient remaining on tissue surface after rinse off.

INCI: Soluble Fibroin

Retention in rinse-off
applications study

Skin Model Activated Silk™ 33B-α 2%

 

Fig 1. Placebo without Activated Silk™ 33B-α

Fig 2. Activated Silk™ 33B-α 2% with Capryl Glucoside

Better Retention.

We conducted clinical trials to test for ongoing positive indications post rinse-off.

Dosage: Body wash with 0.5% Activated Silk™ 33B-α 113 (Commercial body wash)

Length of the study: 7 days

Instrument(s): TEWL and Corneometer

Number of participant(s): 30 subjects

Type of subjects: Healthy male and female subjects aged 18+ with Eczema-prone skin. 50% of the panel had self-perceived sensitive skin.

INCI: Soluble Fibroin

Retention in Rinse-Off
Applications Study: Clinical

Body Wash with Activated Silk™ 33B-α

Results

12% TEWL Improvement

24% Hydration Improvement

In-Vivo Open Label Rinse-off Clinical Study: TEWL
In-Vivo Open Label Rinse-off Clinical Study: Hydration

Better For The Planet.

Not only does Activated Silk™ 33B-α make your skin look and feel healthier, it is also healthier for the planet. Unlike the fossil fuel-derived petrochemicals often used in skincare products, Activated Silk™ 33B-α is sustainable, naturally derived, biodegradable, made with renewable energy and is supportive of a circular economy.

INCI: Soluble Fibroin

Sustainability Claims

  • Naturally Derived
  • Biodegradable
  • Cruelty-Free (Leaping Bunny Certified)
  • Not formulated with Petrochemicals
  • Non-Toxic
  • Made From a Renewable Resource
  • Supportive of a Circular Economy
  • Made With Renewable Energy
  • Reduces Packaging / Shipping Footprint
  • Upcycled

Let’s Make Better Things Together.

INCI: Soluble Fibroin

Data Substantiations (available upon request)

  • 200 Person HRIPT
  • Cytotoxicity in EpiDermFT in Vitro Skin Model
  • 28 Day Clinical Study - Expert Grading
  • 28 Day Clinical Study - Corneometer Measurements
  • 28 Day Clinical Study - Tewameter Measurements